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IHC-plus(tm) PPARG / PPAR Gamma Antibody

LS-B15326
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPPARG
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PPARG / PPAR Gamma Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 21kDa/54kDa/57kDa, while the observed MW by Western blot was 57kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 21kDa/54kDa/57kDa, while the observed MW by Western blot was 57kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5468
  • Target name
    PPARG
  • Target description
    peroxisome proliferator activated receptor gamma
  • Target synonyms
    CIMT1, FPLD3, GLM1, NR1C3, PPARG1, PPARG2, PPARG5, PPARgamma, peroxisome proliferator-activated receptor gamma, PPAR-gamma, nuclear receptor subfamily 1 group C member 3, peroxisome proliferator-activated receptor-gamma 5, peroxisome proliferator-activated receptor-gamma splicing
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PPAR gamma using anti-PPAR gamma antibody (A00449-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPAR gamma antigen affinity purified polyclonal antibody (Catalog # A00449-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPAR gamma at approximately 58 kDa. The expected band size for PPAR gamma is at 58 kDa.
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