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ProductsBack/IHC-plus(tm) PPP3R1 / Calcineurin B Antibody

IHC-plus(tm) PPP3R1 / Calcineurin B Antibody

LS-B13848
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPPP3R1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PPP3R1 / Calcineurin B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 19kDa, while the observed MW by Western blot was 15kDa.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:50 - 1:100), IHC-P (1:100), IP (1:20 - 1:50), WB (1:500 - 1:2000) The predicted MW is 19kDa, while the observed MW by Western blot was 15kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5534
  • Target name
    PPP3R1
  • Target description
    protein phosphatase 3 regulatory subunit B, alpha
  • Target synonyms
    CALNB1, CNB, CNB1, calcineurin subunit B type 1, calcineurin B, type I (19kDa), protein phosphatase 2B regulatory subunit 1, protein phosphatase 2B regulatory subunit B alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PPP3R1 using anti-PPP3R1 antibody (A06469-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPP3R1 antigen affinity purified polyclonal antibody (Catalog # A06469-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PPP3R1 at approximately 15 kDa. The expected band size for PPP3R1 is at 15,19 kDa.
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