IHC-plus(tm) PPP4C Antibody (aa290-301)

LifeSpan BioSciences

IHC-plus(tm) PPP4C Antibody (aa290-301)

23

Immunohistochemistry: LS-B3614 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3614 was determined to be 2.5 ug/ml. Western Blot: Approximately 35kD band observed in HEK293, A431 and HeLa lysates (calculated MW of 35.1kD according to NP_002711.1).. IHC, IHC-P (2.5 µg/ml), Peptide-ELISA (1:16000), WB (0.1 - 0.3 µg/ml) Immunohistochemistry: LS-B3614 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3614 was determined to be 2.5 ug/ml. Western Blot: Approximately 35kD band observed in HEK293, A431 and HeLa lysates (calculated MW of 35.1kD according to NP_002711.1).

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.5 mg/ml

Unconjugated

PPP4C

protein phosphatase 4 catalytic subunit

PP-X, PP4, PP4C, PPH3, PPP4, PPX, serine/threonine-protein phosphatase 4 catalytic subunit, epididymis secretory sperm binding protein, protein phosphatase 4 (formerly X), catalytic subunit, protein phosphatase X, catalytic subunit

Goat

Bovine, Canine, Equine, Hamster, Human, Mammals, Monkey, Mouse, Rabbit, Rat, Xenopus, Zebra Fish

-20°C

41116161