IHC-plus(tm) PRLR / Prolactin Receptor Antibody

LifeSpan BioSciences

IHC-plus(tm) PRLR / Prolactin Receptor Antibody

23

Immunohistochemistry: LS-B2835 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2835 was determined to be 5 ug/ml. Immunoprecipitation: 10 ug/IP reaction.. IHC, IHC-P (5 µg/ml), WB (1 - 3 µg/ml) Immunohistochemistry: LS-B2835 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2835 was determined to be 5 ug/ml. Immunoprecipitation: 10 ug/IP reaction.

Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.25 mg/ml

Unconjugated

PRLR

prolactin receptor

HPRL, MFAB, RI-PRLR, hPRLrI, prolactin receptor, hPRL receptor, secreted prolactin binding protein

Rabbit

Human

-20°C,2°C to 8°C

41116161