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ProductsBack/IHC-plus(tm) PTGES3 / p23 Antibody

IHC-plus(tm) PTGES3 / p23 Antibody

LS-B6976
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
TargetPTGES3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) PTGES3 / p23 Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), WB (1:200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10728
  • Target name
    PTGES3
  • Target description
    prostaglandin E synthase 3
  • Target synonyms
    P23, TEBP, cPGES, prostaglandin E synthase 3, Hsp90 co-chaperone, cytosolic prostaglandin E synthase, cytosolic prostaglandin E2 synthase, progesterone receptor complex p23, prostaglandin E synthase 3 (cytosolic), telomerase-binding protein p23, unactive progesterone receptor, 23 kD
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Bovine, Canine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PTGES3 using anti-PTGES3 antibody (A04136-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human Raji whole cell lysates, Lane 6: human HEK293 whole cell lysates, Lane 7: human K562 whole cell lysates, Lane 8: human THP-1 whole cell lysates, Lane 9: rat PC-12 whole cell lysates, Lane 10: mouse NIH/3T3 whole cell lysates, Lane 11: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGES3 antigen affinity purified polyclonal antibody (Catalog # A04136-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PTGES3 at approximately 23KD. The expected band size for PTGES3 is at 23KD.
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