IHC-plus(tm) RAD52 Antibody
LS-B413
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetRAD52
Overview
- SupplierLifeSpan BioSciences
- Product NameIHC-plus(tm) RAD52 Antibody
- Delivery Days Customer23
- Application Supplier NoteImmunohistochemistry: LS-B413 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B413 was determined to be 2.5 ug/ml.. ELISA (1:5000 - 1:20000), IHC, IHC-P (2.5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B413 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B413 was determined to be 2.5 ug/ml.
- ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Estimated Purity...
- Gene ID5893
- Target nameRAD52
- Target descriptionRAD52 DNA repair protein
- Target synonymsDNA repair protein RAD52 homolog, RAD52 homolog, DNA repair protein, recombination protein RAD52, rhabdomyosarcoma antigen MU-RMS-40.23
- HostRabbit
- IsotypeIgG
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203




![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Rad52 antibody [5H9] (GTX70301) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX70301/GTX70301_44923_20230113_WB_23011618_845.webp)