IHC-plus(tm) RAD52 Antibody

LifeSpan BioSciences

IHC-plus(tm) RAD52 Antibody

23

Immunohistochemistry: LS-B413 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B413 was determined to be 2.5 ug/ml.. ELISA (1:5000 - 1:20000), IHC, IHC-P (2.5 µg/ml), WB (1:500 - 1:2000) Immunohistochemistry: LS-B413 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B413 was determined to be 2.5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

RAD52

RAD52 homolog, DNA repair protein

DNA repair protein RAD52 homolog; recombination protein RAD52; rhabdomyosarcoma antigen MU-RMS-40.23

Rabbit

IgG

Human

-20°C,2°C to 8°C

12352203