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ProductsBack/IHC-plus(tm) Raptor / Mip1 Antibody

IHC-plus(tm) Raptor / Mip1 Antibody

LS-B579
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetRPTOR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) Raptor / Mip1 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 ug/mL. Antibody can also be used for immunocytochemistry starting at 1 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot:. ELISA, ICC, IF, IHC, IHC-P (2.5 µg/ml), WB (2 µg/ml) Raptor antibody can be used for the detection of Raptor by Western blot at 2 and 4 ug/mL. Antibody can also be used for immunocytochemistry starting at 1 ug/mL. For immunofluorescence start at 10 ug/mL. Antibody validated: Western Blot in mouse samples; Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57521
  • Target name
    RPTOR
  • Target description
    regulatory associated protein of MTOR complex 1
  • Target synonyms
    KOG1, Mip1, regulatory-associated protein of mTOR, p150 target of rapamycin (TOR)-scaffold protein containing WD-repeats, raptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Raptor/RPTOR using anti-Raptor/RPTOR antibody (A01463-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Mcf-7 whole cell lysates, Lane 4: human Hek293 whole cell lysates, Lane 5: human A549 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Raptor/RPTOR antigen affinity purified polyclonal antibody (Catalog # A01463-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Raptor/RPTOR at approximately 150KD. The expected band size for Raptor/RPTOR is at 150KD.
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