IHC-plus(tm) RFFL Antibody

LifeSpan BioSciences

IHC-plus(tm) RFFL Antibody

23

Immunohistochemistry: LS-B416 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B416 was determined to be 5 ug/ml.. ELISA (1:4000 - 1:20000), IHC, IHC-P (5 µg/ml), WB (1:500 - 1:3000) Immunohistochemistry: LS-B416 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B416 was determined to be 5 ug/ml.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1 mg/ml

Unconjugated

Lyophilized

RFFL

ring finger and FYVE like domain containing E3 ubiquitin protein ligase

CARP2; CARP-2; caspase 8 and 10 associated RING protein-2; caspase regulator CARP2; caspases-8 and -10-associated RING finger protein 2; E3 ubiquitin-protein ligase rififylin; FRING; FYVE-RING finger protein SAKURA; RIFIFYLIN; ring finger and FYVE-like domain containing 1; RING finger and FYVE-like domain-containing protein 1; RING finger protein 189; RING-type E3 ubiquitin transferase rififylin; RNF189; RNF34L

Rabbit

IgG

Human

2°C to 8°C or -20°C

12352203