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ProductsBack/IHC-plus(tm) RNF20 Antibody

IHC-plus(tm) RNF20 Antibody

LS-B10108
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetRNF20
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) RNF20 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    RNF20 antibody can be used for detection of RNF20 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 99, 107 kDa Observed: 100, 110 kDa. ELISA, IF (20 µg/ml), IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml) RNF20 antibody can be used for detection of RNF20 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 99, 107 kDa Observed: 100, 110 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID56254
  • Target name
    RNF20
  • Target description
    ring finger protein 20
  • Target synonyms
    BRE1, BRE1A, hBRE1, E3 ubiquitin-protein ligase BRE1A, BRE1 E3 ubiquitin ligase homolog, RING-type E3 ubiquitin transferase BRE1A, homolog of S. cerevisiae BRE1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RNF20 using anti-RNF20 antibody (A03457-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: rat liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF20 antigen affinity purified polyclonal antibody (Catalog # A03457-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RNF20 at approximately 114 kDa. The expected band size for RNF20 is at 114 kDa.
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