IHC-plus(tm) RNH1 Antibody (clone AT1H23)

LifeSpan BioSciences

IHC-plus(tm) RNH1 Antibody (clone AT1H23)

23

Immunohistochemistry: LS-B3585 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3585 was determined to be 5 ug/ml. Western Blot: Extracts of mouse liver (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RNH1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.. ELISA, IHC, IHC-P (5 µg/ml), WB (1:1000 - 1:2000) Immunohistochemistry: LS-B3585 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3585 was determined to be 5 ug/ml. Western Blot: Extracts of mouse liver (20ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human RNH1 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

AT1H23

1 mg/ml

Unconjugated

RNH1

ribonuclease/angiogenin inhibitor 1

IIAE12, RAI, RNH, ribonuclease inhibitor, placental RNase inhibitor, placental ribonuclease inhibitor, testicular tissue protein Li 164

Mouse

IgG2a

Human, Mouse

-20°C

41116161