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ProductsBack/IHC-plus(tm) RPL27 / Ribosomal Protein L27 Antibody

IHC-plus(tm) RPL27 / Ribosomal Protein L27 Antibody

LS-B11257
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetRPL27
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) RPL27 / Ribosomal Protein L27 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Recommended conditions for Immunohistochemistry: For FFPE tissues, perform steam-based antigen retrieval using Tris/EDTA buffer at pH 9.0.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (20 µg/ml), WB Recommended conditions for Immunohistochemistry: For FFPE tissues, perform steam-based antigen retrieval using Tris/EDTA buffer at pH 9.0.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6155
  • Target name
    RPL27
  • Target description
    ribosomal protein L27
  • Target synonyms
    DBA16, L27, eL27, large ribosomal subunit protein eL27, 60S ribosomal protein L27
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RPL27 using anti-RPL27 antibody (A09736-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human RT4 whole cell lysates, Lane 6: human Hela whole cell lysates, Lane 8: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RPL27 antigen affinity purified polyclonal antibody (Catalog # A09736-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RPL27 at approximately 16 kDa. The expected band size for RPL27 is at 16 kDa.
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