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ProductsBack/IHC-plus(tm) RSL24D1 Antibody (clone 2A10-1E5)

IHC-plus(tm) RSL24D1 Antibody (clone 2A10-1E5)

LS-B6227
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetRSL24D1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) RSL24D1 Antibody (clone 2A10-1E5)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Recombinant protein.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IF (10 µg/ml), IHC, IHC-P (1 - 5 µg/ml), WB (1:500 - 1:1000) Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Western Blot: Recombinant protein.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2A10-1E5
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID51187
  • Target name
    RSL24D1
  • Target description
    ribosomal L24 domain containing 1
  • Target synonyms
    C15orf15, HRP-L30-iso, L30, RLP24, RPL24, RPL24L, TVAS3, probable ribosome biogenesis protein RLP24, 60S ribosomal protein L30 isolog, homolog of yeast ribosomal like protein 24, my024 protein, ribosomal L24 domain-containing protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of RSL24D1 using anti-RSL24D1 antibody (A14856-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human PC-3 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: rat RH35 whole cell lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse RAW264.7 whole cell lysates, Lane 8: mouse SP2/0 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RSL24D1 antigen affinity purified polyclonal antibody (Catalog # A14856-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RSL24D1 at approximately 20 kDa. The expected band size for RSL24D1 is at 20 kDa.
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