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ProductsBack/IHC-plus(tm) RTCB / C22orf28 Antibody (aa201-214)

IHC-plus(tm) RTCB / C22orf28 Antibody (aa201-214)

LS-B10474
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Equine, Human, Mammals, Porcine
TargetRTCB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) RTCB / C22orf28 Antibody (aa201-214)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 55kD band observed in lysates of cell lines HeLa, Jurkat and K562 (calculated MW of 55.2kD according to NP_055121.1). Recommended concentration: 0.1-0.3 ug/ml.. IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:64000), WB (0.1 - 0.3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:64000. Western blot: Approx 55kD band observed in lysates of cell lines HeLa, Jurkat and K562 (calculated MW of 55.2kD according to NP_055121.1). Recommended concentration: 0.1-0.3 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51493
  • Target name
    RTCB
  • Target description
    RNA 2',3'-cyclic phosphate and 5'-OH ligase
  • Target synonyms
    C22orf28, DJ149A16.6, FAAP, HSPC117, RNA-splicing ligase RtcB homolog, 3'-phosphate/5'-hydroxy nucleic acid ligase, ankyrin repeat domain 54, focal adhesion-associated protein, tRNA-splicing ligase RtcB homolog
  • Host
    Goat
  • Reactivity
    Canine, Equine, Human, Mammals, Porcine
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of RTCB using anti-RTCB antibody (A05076-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RTCB antigen affinity purified polyclonal antibody (Catalog # A05076-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RTCB at approximately 55 kDa. The expected band size for RTCB is at 55 kDa.
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