IHC-plus(tm) S100A8 / MRP8 Antibody (FITC)

LifeSpan BioSciences

IHC-plus(tm) S100A8 / MRP8 Antibody (FITC)

23

Immunohistochemistry: LS-B2247 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2247 was determined to be 1:50. Flow Cytometry: Neat. Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is required. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.. Flo, IHC, IHC-P (1:50) Immunohistochemistry: LS-B2247 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2247 was determined to be 1:50. Flow Cytometry: Neat. Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul. Membrane permeabilization is required. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

0.1 mg/ml

FITC

S100A8

S100 calcium binding protein A8

60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8, S100-A8, protein S100-A8, calgranulin A, calprotectin L1L subunit, cystic fibrosis antigen, leukocyte L1 complex light chain, migration inhibitory factor-related protein 8, urinary stone protein band A

Mouse

IgG1

Bovine, Canine, Equine, Feline, Human, Monkey, Porcine, Rabbit, Rat

-20°C,2°C to 8°C

41116161