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ProductsBack/IHC-plus(tm) SENP6 Antibody (aa1081-1112)

IHC-plus(tm) SENP6 Antibody (aa1081-1112)

LS-B13740
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSENP6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SENP6 Antibody (aa1081-1112)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:50 - 1:100), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID26054
  • Target name
    SENP6
  • Target description
    SUMO specific peptidase 6
  • Target synonyms
    SSP1, SUSP1, sentrin-specific protease 6, 2810017C20Rik, SUMO-1-specific protease 1, SUMO1/sentrin specific peptidase 6, SUMO1/sentrin specific protease 6, sentrin/SUMO-specific protease SENP6
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SENP6 using anti-SENP6 antibody (A05088-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SENP6 antigen affinity purified polyclonal antibody (Catalog # A05088-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SENP6 at approximately 150KD. The expected band size for SENP6 is at 126KD.
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