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ProductsBack/IHC-plus(tm) SFRP4 Antibody (aa239-253)

IHC-plus(tm) SFRP4 Antibody (aa239-253)

LS-B5904
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
TargetSFRP4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SFRP4 Antibody (aa239-253)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 40kDa band observed in Human Uterus lysates (calculated MW of 39.8kDa according to NP_003005.2). Recommended concentration: 0.3-1 ug/ml.. IHC, IHC-P (3.75 µg/ml), Peptide-ELISA (1:32000), WB (1 - 3 µg/ml) Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx. 40kDa band observed in Human Uterus lysates (calculated MW of 39.8kDa according to NP_003005.2). Recommended concentration: 0.3-1 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6424
  • Target name
    SFRP4
  • Target description
    secreted frizzled related protein 4
  • Target synonyms
    FRP-4, FRPHE, FRZB-2, PYL, sFRP-4, secreted frizzled-related protein 4, frizzled protein, human endometrium, secreted frizzled-related protein 4; secreted frizzled-related protein 4
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Equine, Hamster, Human, Mammals, Monkey, Mouse, Porcine, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SFRP4 using anti-SFRP4 antibody (PB9888). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: A549 whole cell lysates, Lane 2: SW620 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SFRP4 antigen affinity purified polyclonal antibody (Catalog # PB9888) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SFRP4 at approximately 49 kDa. The expected band size for SFRP4 is at 40 kDa.
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