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IHC-plus(tm) SIRT3 / Sirtuin 3 Antibody

LS-B13905
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSIRT3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SIRT3 / Sirtuin 3 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 28kDa/43kDa, while the observed MW by Western blot was 27kDa/44kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:100), WB The predicted MW is 28kDa/43kDa, while the observed MW by Western blot was 27kDa/44kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID23410
  • Target name
    SIRT3
  • Target description
    sirtuin 3
  • Target synonyms
    SIR2L3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, NAD-dependent deacetylase sirtuin-3, mitochondrial, NAD-dependent protein delactylase sirtuin-3, SIR2-like protein 3, mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase, regulatory protein SIR2 homolog 3, silent mating type information regulation 2, S.cerevisiae, homolog 3, sir2-like 3, sirtuin type 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SIRT3 using anti-SIRT3 antibody (A01061-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hacat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT3 antigen affinity purified polyclonal antibody (Catalog # A01061-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT3 at approximately 43 kDa. The expected band size for SIRT3 is at 43 kDa.
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