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ProductsBack/IHC-plus(tm) SKIL / SNO / SnoN Antibody (clone 2F6)

IHC-plus(tm) SKIL / SNO / SnoN Antibody (clone 2F6)

LS-B12192
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetSKIL
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SKIL / SNO / SnoN Antibody (clone 2F6)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (10 µg/ml), WB (1:500 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2F6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6498
  • Target name
    SKIL
  • Target description
    SKI like proto-oncogene
  • Target synonyms
    SNO, SnoA, SnoI, SnoN, ski-like protein, SKI-like oncogene, ski-related oncogene snoN
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SnoN/SNO/SKIL using anti-SnoN/SNO/SKIL antibody (A04131-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Sw620 whole cell lysates, Lane 2: human HT1080 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: rat L6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SnoN/SNO/SKIL antigen affinity purified polyclonal antibody (Catalog # A04131-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SnoN/SNO/SKIL at approximately 77 kDa. The expected band size for SnoN/SNO/SKIL is at 77 kDa.
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