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ProductsBack/IHC-plus(tm) SLC18A3 / VACHT Antibody

IHC-plus(tm) SLC18A3 / VACHT Antibody

LS-B16846
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSLC18A3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SLC18A3 / VACHT Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    IHC: Heat treatment is necessary for paraffin-embedded sections;
  • Applications
    Flow Cytometry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo, IHC-P (1:100) IHC: Heat treatment is necessary for paraffin-embedded sections;
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID6572
  • Target name
    SLC18A3
  • Target description
    solute carrier family 18 member A3
  • Target synonyms
    CMS21, VACHT, vesicular acetylcholine transporter, solute carrier family 18 (vesicular acetylcholine transporter), member 3, solute carrier family 18 (vesicular acetylcholine), member 3, solute carrier family 18, member 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SLC18A3 using anti-SLC18A3 antibody (A04891). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. lane 1: HELA whole cell lysates, lane 2: HEPA whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SLC18A3 antigen affinity purified polyclonal antibody (Catalog # A04891) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SLC18A3 at approximately 55KD. The expected band size for SLC18A3 is at 57KD.
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