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ProductsBack/IHC-plus(tm) SLC22A6 / OAT1 Antibody (aa513-541)

IHC-plus(tm) SLC22A6 / OAT1 Antibody (aa513-541)

LS-B10034
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSLC22A6
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SLC22A6 / OAT1 Antibody (aa513-541)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:10 - 1:50), IHC, IHC-P (1:50), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.37 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9356
  • Target name
    SLC22A6
  • Target description
    solute carrier family 22 member 6
  • Target synonyms
    HOAT1, OAT1, PAHT, ROAT1, solute carrier family 22 member 6, PAH transporter, hPAHT, hROAT1, organic anion transporter 1, para-aminohippurate transporter, renal organic anion transporter 1, solute carrier family 22 (organic anion transporter), member 6
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OAT1/SLC22A6 using anti-OAT1/SLC22A6 antibody (A02456-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OAT1/SLC22A6 antigen affinity purified polyclonal antibody (Catalog # A02456-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OAT1/SLC22A6 at approximately 70 kDa. The expected band size for OAT1/SLC22A6 is at 62 kDa.
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