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ProductsBack/IHC-plus(tm) SMG9 Antibody (aa75-125)

IHC-plus(tm) SMG9 Antibody (aa75-125)

LS-B14077
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSMG9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SMG9 Antibody (aa75-125)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Applications
    ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), IP (5 - 10 µg/mg lysate) IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56006
  • Target name
    SMG9
  • Target description
    SMG9 nonsense mediated mRNA decay factor
  • Target synonyms
    C19orf61, F17127_1, HBMS, NEDITPO, nonsense-mediated mRNA decay factor SMG9, protein smg-9 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of C19orf61/SMG9 using anti-C19orf61/SMG9 antibody (A10233-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human MOLT-4 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat testis tissue lysates, Lane 6: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-C19orf61/SMG9 antigen affinity purified polyclonal antibody (Catalog # A10233-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for C19orf61/SMG9 at approximately 58 kDa. The expected band size for C19orf61/SMG9 is at 57-65 kDa.
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