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ProductsBack/IHC-plus(tm) SSB / La Antibody (clone 1E11)

IHC-plus(tm) SSB / La Antibody (clone 1E11)

LS-B10046
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetSSB
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) SSB / La Antibody (clone 1E11)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:100), IF (1:50 - 1:100), IHC, IHC-P (10 µg/ml), IP, WB (1:1000 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    1E11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6741
  • Target name
    SSB
  • Target description
    small RNA binding exonuclease protection factor La
  • Target synonyms
    LARP3, La, La/SSB, lupus La protein, La ribonucleoprotein domain family, member 3, SS-B, SS-B/La protein, Sjogren syndrome antigen B, autoantigen La, la autoantigen, lupus La antigen, sjoegren syndrome type B antigen
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Canine, Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SSB using anti-SSB antibody (A00705-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HEL whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSB antigen affinity purified polyclonal antibody (Catalog # A00705-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SSB at approximately 47 kDa. The expected band size for SSB is at 47 kDa.
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