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ProductsBack/IHC-plus(tm) STX18 / Syntaxin 18 Antibody (clone 2E5)

IHC-plus(tm) STX18 / Syntaxin 18 Antibody (clone 2E5)

LS-B6350
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSTX18
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) STX18 / Syntaxin 18 Antibody (clone 2E5)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Western Blot using cell line lysates and the recombinant protein used as the immunogen.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (5 µg/ml), WB (1 - 5 µg/ml) Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Western Blot using cell line lysates and the recombinant protein used as the immunogen.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2E5
  • Conjugate
    Unconjugated
  • Gene ID53407
  • Target name
    STX18
  • Target description
    syntaxin 18
  • Target synonyms
    Ufe1, syntaxin-18, cell growth-inhibiting gene 9 protein
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Syntaxin 18/STX18 using anti-Syntaxin 18/STX18 antibody (A11895). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Syntaxin 18/STX18 antigen affinity purified polyclonal antibody (Catalog # A11895) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Syntaxin 18/STX18 at approximately 39 kDa. The expected band size for Syntaxin 18/STX18 is at 39 kDa.
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