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ProductsBack/IHC-plus(tm) TFPI / LACI Antibody

IHC-plus(tm) TFPI / LACI Antibody

LS-B10121
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTFPI
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TFPI / LACI Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    TFPI antibody can be used for detection of TFPI by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Western Blot: Predicted: 33 kDa Observed: 30 kDa. ELISA, IF (20 µg/ml), IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml) TFPI antibody can be used for detection of TFPI by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested. Western Blot: Predicted: 33 kDa Observed: 30 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7035
  • Target name
    TFPI
  • Target description
    tissue factor pathway inhibitor
  • Target synonyms
    EPI, LACI, TFI, TFPI1, tissue factor pathway inhibitor, anti-convertin, extrinsic pathway inhibitor, tissue factor pathway inhibitor (lipoprotein-associated coagulation inhibitor)
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TFPI using anti-TFPI antibody (A01052). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: HELA whole Cell lysates, Lane 2: MCF-7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TFPI antigen affinity purified polyclonal antibody (Catalog # A01052) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TFPI at approximately 45KD. The expected band size for TFPI is at 35KD.
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