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ProductsBack/IHC-plus(tm) TJP2 / ZO2 / ZO-2 Antibody

IHC-plus(tm) TJP2 / ZO2 / ZO-2 Antibody

LS-B14372
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTJP2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TJP2 / ZO2 / ZO-2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 111-137kDa, while the observed MW by Western blot was 160kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (1:200), WB (1:500 - 1:2000) The predicted MW is 111-137kDa, while the observed MW by Western blot was 160kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9414
  • Target name
    TJP2
  • Target description
    tight junction protein 2
  • Target synonyms
    C9DUPq21.11, DFNA51, DUP9q21.11, FHCA1, PFIC4, X104, ZO2, tight junction protein 2, Friedreich ataxia region gene X104 (tight junction protein ZO-2), zona occludens 2, zonula occludens protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TJP2/ZO2 using anti-TJP2/ZO2 antibody (A02774-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hek293 whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TJP2/ZO2 antigen affinity purified polyclonal antibody (Catalog # A02774-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TJP2/ZO2 at approximately 150KD. The expected band size for TJP2/ZO2 is at 150KD.
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