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IHC-plus(tm) TM9SF3 Antibody (Internal)

LS-A8165
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus
TargetTM9SF3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TM9SF3 Antibody (Internal)
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (10 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56889
  • Target name
    TM9SF3
  • Target description
    transmembrane 9 superfamily member 3
  • Target synonyms
    EP70-P-iso, SMBP, transmembrane 9 superfamily member 3, SM-11044-binding protein, dinucleotide oxidase disulfide thiol exchanger 3 superfamily member 3, endomembrane protein emp70 precursor isolog
  • Host
    Rabbit
  • Reactivity
    Bovine, Canine, Chicken, Equine, Guinea Pig, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Xenopus
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TM9SF3 using anti-TM9SF3 antibody (A12868-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human A431 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TM9SF3 antigen affinity purified polyclonal antibody (Catalog # A12868-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TM9SF3 at approximately 72 kDa. The expected band size for TM9SF3 is at 68 kDa.
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