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ProductsBack/IHC-plus(tm) TMPRSS3 Antibody (aa405-454)

IHC-plus(tm) TMPRSS3 Antibody (aa405-454)

LS-B9928
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTMPRSS3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TMPRSS3 Antibody (aa405-454)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Predicted MW 49 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:100 - 1:500), IHC, IHC-P (1:100), Peptide-ELISA (1:10000), WB (1:500 - 1:1000) Western Blot: Predicted MW 49 kDa
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64699
  • Target name
    TMPRSS3
  • Target description
    transmembrane serine protease 3
  • Target synonyms
    DFNB10, DFNB8, ECHOS1, TADG12, transmembrane protease serine 3, serine protease TADG-12, transmembrane protease, serine 3, tumor-associated differentially-expressed gene 12 protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TMPRSS3 using anti-TMPRSS3 antibody (A04164-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: rat pancreas tissue lysates, Lane 4: rat stomach tissue lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TMPRSS3 antigen affinity purified polyclonal antibody (Catalog # A04164-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TMPRSS3 at approximately 48KD. The expected band size for TMPRSS3 is at 48KD.
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