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ProductsBack/IHC-plus(tm) TNFRSF12A / TWEAK Receptor Antibody

IHC-plus(tm) TNFRSF12A / TWEAK Receptor Antibody

LS-B605
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTNFRSF12A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TNFRSF12A / TWEAK Receptor Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Fn14 antibody can be used for the detection of Fn14 by Western blot at 2 - 4 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:. ELISA, IF, IHC, IHC-P (2.5 µg/ml), WB (1 µg/ml) Fn14 antibody can be used for the detection of Fn14 by Western blot at 2 - 4 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot:
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51330
  • Target name
    TNFRSF12A
  • Target description
    TNF receptor superfamily member 12A
  • Target synonyms
    CD266, FN14, TWEAKR, tumor necrosis factor receptor superfamily member 12A, FGF-inducible 14, fibroblast growth factor-inducible immediate-early response protein 14, tweak-receptor, type I transmembrane protein Fn14
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TWEAKR using anti-TWEAKR antibody (M06261). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HUVEC whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse NIH/3T3 whole cell lysates, Lane 6: mouse 4T1 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TWEAKR antigen affinity purified monoclonal antibody (Catalog # M06261) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TWEAKR at approximately 17,20 kDa. The expected band size for TWEAKR is at 17,20 kDa.
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