IHC-plus(tm) TNFRSF1B / TNFR2 Antibody

LifeSpan BioSciences

IHC-plus(tm) TNFRSF1B / TNFR2 Antibody

23

Immunohistochemistry: LS-B7424 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human sTNF Receptor-II (0.5 ug/ml) a concentration of 15-20 ug/ml of this antibody is required. ELISA: To detect hsTNF-receptor II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTNF-receptor II (LS-C104278) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTNF-receptor II. Western Blot: To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7424 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human sTNF Receptor-II (0.5 ug/ml) a concentration of 15-20 ug/ml of this antibody is required. ELISA: To detect hsTNF-receptor II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sTNF-receptor II (LS-C104278) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTNF-receptor II. Western Blot: To detect hsTNF-receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF-receptor II is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

TNFRSF1B

TNF receptor superfamily member 1B

CD120b, TBPII, TNF-R-II, TNF-R75, TNFBR, TNFR1B, TNFR2, TNFR80, p75, p75TNFR, tumor necrosis factor receptor superfamily member 1B, TNF-R2, TNF-RII, p75 TNF receptor, p80 TNF-alpha receptor, tumor necrosis factor beta receptor, tumor necrosis factor binding protein 2, tumor necrosis factor receptor 2, tumor necrosis factor receptor type II

Rabbit

IgG

Human

-20°C,2°C to 8°C,RT

41116161