IHC-plus(tm) TNFSF11 / RANKL / TRANCE Antibody

LifeSpan BioSciences

IHC-plus(tm) TNFSF11 / RANKL / TRANCE Antibody

23

Immunohistochemistry: LS-B7419 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hsRANKL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sRANKL (LS-C104397) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANKL. Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7419 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. ELISA: To detect hsRANKL by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human sRANKL (LS-C104397) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsRANKL. Western Blot: To detect hsRANKL by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsRANKL is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

TNFSF11

TNF superfamily member 11

CD254, ODF, OPGL, OPTB2, RANKL, TNLG6B, TRANCE, hRANKL2, sOdf, tumor necrosis factor ligand superfamily member 11, TNF-related activation-induced cytokine, osteoclast differentiation factor, osteoprotegerin ligand, receptor activator of nuclear factor kappa B ligand, tumor necrosis factor (ligand) superfamily, member 11, tumor necrosis factor ligand 6B, tumor necrosis factor superfamily member 11

Goat

IgG

Human

-20°C,2°C to 8°C,RT

41116161