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ProductsBack/IHC-plus(tm) TNFSF15 / TL1A / VEGI Antibody

IHC-plus(tm) TNFSF15 / TL1A / VEGI Antibody

LS-B2406
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTNFSF15
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TNFSF15 / TL1A / VEGI Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    TL1A antibody can be used for detection of TL1A by Western blot at 0.5 to 2 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot: Predicted: 28 kDa Observed: 26 kDa. ELISA, IHC, IHC-P (5 µg/ml), WB (0.5 - 1 µg/ml) TL1A antibody can be used for detection of TL1A by Western blot at 0.5 to 2 ug/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Western Blot: Predicted: 28 kDa Observed: 26 kDa
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9966
  • Target name
    TNFSF15
  • Target description
    TNF superfamily member 15
  • Target synonyms
    TL1, TL1A, TNLG1B, VEGI, VEGI192A, tumor necrosis factor ligand superfamily member 15, TNF ligand-related molecule 1, TNF superfamily ligand TL1A, tumor necrosis factor (ligand) superfamily, member 15, tumor necrosis factor ligand 1B, tumor necrosis factor superfamily member 15, vascular endothelial cell growth inhibitor, vascular endothelial growth inhibitor, vascular endothelial growth inhibitor-192A
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TL1A using anti-TL1A antibody (A02402-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: rat kidney tissue lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TL1A antigen affinity purified polyclonal antibody (Catalog # A02402-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TL1A at approximately 28KD. The expected band size for TL1A is at 28KD.
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