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ProductsBack/IHC-plus(tm) TPH1 / Tryptophan Hydroxylase Antibody

IHC-plus(tm) TPH1 / Tryptophan Hydroxylase Antibody

LS-B14377
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTPH1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TPH1 / Tryptophan Hydroxylase Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 50kDa/53kDa, while the observed MW by Western blot was 50kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:50 - 1:200), IHC-P (1:100 - 1:200), WB (1:500 - 1:2000) The predicted MW is 50kDa/53kDa, while the observed MW by Western blot was 50kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.135 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7166
  • Target name
    TPH1
  • Target description
    tryptophan hydroxylase 1
  • Target synonyms
    TPRH, TRPH, tryptophan 5-hydroxylase 1, L-tryptophan hydroxylase, indoleacetic acid-5-hydroxylase, tryptophan 5-monooxygenase 1, tryptophan hydroxylase (tryptophan 5-monooxygenase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of Tryptophan Hydroxylase using anti-Tryptophan Hydroxylase antibody (A01626-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human SGC-7901 whole cell lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse heart tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Tryptophan Hydroxylase antigen affinity purified polyclonal antibody (Catalog # A01626-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Tryptophan Hydroxylase at approximately 51KD. The expected band size for Tryptophan Hydroxylase is at 51KD.
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