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ProductsBack/IHC-plus(tm) TPR Antibody (aa2300-C-term)

IHC-plus(tm) TPR Antibody (aa2300-C-term)

LS-B14268
LifeSpan BioSciences
ApplicationsImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetTPR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TPR Antibody (aa2300-C-term)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC-IF: 1:100-1:500
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ICC (1:100 - 1:500), IF (1:50 - 1:500), IHC, IHC-P (1:100) IHC-IF: 1:100-1:500
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID7175
  • Target name
    TPR
  • Target description
    translocated promoter region, nuclear basket protein
  • Target synonyms
    MRT79, nucleoprotein TPR, NPC-associated intranuclear protein, megator, nuclear pore complex-associated protein TPR, translocated promoter region (to activated MET oncogene), translocated promoter region protein, tumor potentiating region
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of TPR using anti-TPR antibody (A00695-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HL-60 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TPR antigen affinity purified polyclonal antibody (Catalog # A00695-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TPR at approximately 300 kDa. The expected band size for TPR is at 265 kDa.
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