IHC-plus(tm) TRAF6 Antibody (C-Terminus)

LifeSpan BioSciences

IHC-plus(tm) TRAF6 Antibody (C-Terminus)

23

Immunohistochemistry: LS-B190 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B190 was determined to be 2.5 ug/ml. Immunoprecipitation: 7 ug/reaction. Positive control: Human Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates. Please note that cross-reactivity of this antibody with mouse has been confirmed only in WB. The antibody has not been evaluated for use in IHC with mouse samples at this time and suitability cannot be guaranteed.. IHC, IHC-P (1:200 - 1:400), IP, WB (1 - 3 µg/ml) Immunohistochemistry: LS-B190 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B190 was determined to be 2.5 ug/ml. Immunoprecipitation: 7 ug/reaction. Positive control: Human Hela, HEK293, Jurkat, and mouse NIH3T3 cell lysates. Please note that cross-reactivity of this antibody with mouse has been confirmed only in WB. The antibody has not been evaluated for use in IHC with mouse samples at this time and suitability cannot be guaranteed.

ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

0.25 mg/ml

Unconjugated

TRAF6

TNF receptor associated factor 6

MGC:3310, RNF85, TNF receptor-associated factor 6, E3 ubiquitin-protein ligase TRAF6, RING finger protein 85, RING-type E3 ubiquitin transferase TRAF6, TNF receptor-associated factor 6, E3 ubiquitin protein ligase, interleukin-1 signal transducer

Rabbit

IgG

Human, Mouse

-20°C,2°C to 8°C

41116161