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ProductsBack/IHC-plus(tm) TRIM25 Antibody (clone 5C3)

IHC-plus(tm) TRIM25 Antibody (clone 5C3)

LS-B12178
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTRIM25
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TRIM25 Antibody (clone 5C3)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IF (20 µg/ml), IHC, IHC-P (3 - 10 µg/ml), WB Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5C3
  • Conjugate
    Unconjugated
  • Gene ID7706
  • Target name
    TRIM25
  • Target description
    tripartite motif containing 25
  • Target synonyms
    EFP, RNF147, Z147, ZNF147, E3 ubiquitin/ISG15 ligase TRIM25, RING finger protein 147, RING-type E3 ubiquitin transferase TRIM25, estrogen-responsive finger protein, tripartite motif protein TRIM25, tripartite motif-containing protein 25, ubiquitin/ISG15-conjugating enzyme TRIM25, zinc finger protein 147 (estrogen-responsive finger protein), zinc finger protein-147
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of TRIM25 using anti-TRIM25 antibody (A03232-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human T-47D whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM25 antigen affinity purified polyclonal antibody (Catalog # A03232-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM25 at approximately 71KD. The expected band size for TRIM25 is at 71KD.
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