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ProductsBack/IHC-plus(tm) TRIM3 Antibody (aa1-50)

IHC-plus(tm) TRIM3 Antibody (aa1-50)

LS-B12212
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTRIM3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) TRIM3 Antibody (aa1-50)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Applications
    ImmunoPrecipitation, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (7.5 µg/ml), IP (2 - 5 µg/mg lysate) IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10612
  • Target name
    TRIM3
  • Target description
    tripartite motif containing 3
  • Target synonyms
    BERP, HAC1, RNF22, RNF97, tripartite motif-containing protein 3, RING finger protein 97, brain expressed ring finger, brain-expressed RING finger protein, ring finger protein 22, tripartite motif protein TRIM3
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of TRIM3 using anti-TRIM3 antibody (A07081-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human T-47D whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM3 antigen affinity purified polyclonal antibody (Catalog # A07081-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TRIM3 at approximately 81 kDa. The expected band size for TRIM3 is at 81 kDa.
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