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ProductsBack/IHC-plus(tm) USP15 Antibody (aa960-C-term)

IHC-plus(tm) USP15 Antibody (aa960-C-term)

LS-B14420
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetUSP15
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) USP15 Antibody (aa960-C-term)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Efficiency of IP is low. IHC: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), IP (2 - 5 µg/mg lysate), WB (1:1000 - 1:5000) Efficiency of IP is low. IHC: Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9958
  • Target name
    USP15
  • Target description
    ubiquitin specific peptidase 15
  • Target synonyms
    UNPH-2, UNPH4, ubiquitin carboxyl-terminal hydrolase 15, deubiquitinating enzyme 15, ubiquitin thioesterase 15, ubiquitin thiolesterase 15, ubiquitin-specific-processing protease 15
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of USP15 using anti-USP15 antibody (A03057-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-USP15 antigen affinity purified polyclonal antibody (Catalog # A03057-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for USP15 at approximately 112KD. The expected band size for USP15 is at 112KD.
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