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IHC-plus(tm) VCP Antibody (Internal Region)

LS-B16968
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetVCP
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) VCP Antibody (Internal Region)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 100kDa band observed in lysates of cell line HeLa (calculated MW of 92.0kDa according to NP_010157.1). Recommended concentration: 0.01-0.03 ug/ml.. IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.01 - 0.03 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 100kDa band observed in lysates of cell line HeLa (calculated MW of 92.0kDa according to NP_010157.1). Recommended concentration: 0.01-0.03 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID7415
  • Target name
    VCP
  • Target description
    valosin containing protein
  • Target synonyms
    CDC48, FTDALS6, TERA, p97, transitional endoplasmic reticulum ATPase, 15S Mg(2+)-ATPase p97 subunit, TER ATPase
  • Host
    Goat
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of VCP using anti-VCP antibody (A00610-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human A431 whole cell lysates, Lane 5: Monkey COS-7 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human placenta tissue lysates, Lane 8: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VCP antigen affinity purified polyclonal antibody (Catalog # A00610-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VCP at approximately 97 kDa. The expected band size for VCP is at 89 kDa.
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