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ProductsBack/IHC-plus(tm) VTN / Vitronectin Antibody

IHC-plus(tm) VTN / Vitronectin Antibody

LS-B7842
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetVTN
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) VTN / Vitronectin Antibody
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-P (1:200), WB (1:5000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID7448
  • Target name
    VTN
  • Target description
    vitronectin
  • Target synonyms
    V75, VN, VNT, vitronectin, complement S-protein, epibolin, serum spreading factor, somatomedin B
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Vitronectin using anti-Vitronectin antibody (PB9458). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Liver Tissue Lysate at 50ug, Lane 2: Rat Lung Tissue Lysate at 50ug, Lane 3: HEPG2 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug, Lane 5: HEPA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Vitronectin antigen affinity purified polyclonal antibody (Catalog # PB9458) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Vitronectin at approximately 54 kDa. The expected band size for Vitronectin is at 54 kDa.
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