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ProductsBack/IHC-plus(tm) WDR5 Antibody (aa50-100)

IHC-plus(tm) WDR5 Antibody (aa50-100)

LS-B14433
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetWDR5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) WDR5 Antibody (aa50-100)
  • Delivery Days Customer
    23
  • Application Supplier Note
    IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC, IHC-P (5 µg/ml), WB (1:2000 - 1:10000) IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. IP
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11091
  • Target name
    WDR5
  • Target description
    WD repeat domain 5
  • Target synonyms
    BIG-3, BIG3, CFAP89, SWD3, WD repeat-containing protein 5, BMP-2-induced gene 3 kb, BMP2-induced 3-kb gene protein, SWD3, Set1c WD40 repeat protein, homolog, cilia and flagella associated protein 89
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of WDR5 using anti-WDR5 antibody (A01910-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-WDR5 antigen affinity purified polyclonal antibody (Catalog # A01910-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for WDR5 at approximately 37 kDa. The expected band size for WDR5 is at 37 kDa.
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