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ProductsBack/IHC-plus(tm) ZC3H12A / MCPIP1 Antibody (aa331-358)

IHC-plus(tm) ZC3H12A / MCPIP1 Antibody (aa331-358)

LS-B11221
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetZC3H12A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IHC-plus(tm) ZC3H12A / MCPIP1 Antibody (aa331-358)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:10 - 1:50), IHC, IHC-P (1:100), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID80149
  • Target name
    ZC3H12A
  • Target description
    zinc finger CCCH-type containing 12A
  • Target synonyms
    MCPIP, MCPIP-1, MCPIP1, Reg1, dJ423B22.1, endoribonuclease ZC3H12A, MCP-1 treatment-induced protein, MCP-induced protein 1, bifunctional endoribonuclease and deubiquitinase ZC3H12A, monocyte chemotactic protein-induced protein 1, regnase-1, ribonuclease ZC3H12A, zinc finger CCCH domain-containing protein 12A
  • Host
    Rabbit
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MCPIP1/ZC3H12A using anti-MCPIP1/ZC3H12A antibody (A01688-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human Sw620 whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 4: rat spleen tissue lysates, Lane 5: rat kidney tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCPIP1/ZC3H12A antigen affinity purified polyclonal antibody (Catalog # A01688-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MCPIP1/ZC3H12A at approximately 73KD. The expected band size for MCPIP1/ZC3H12A is at 66KD.
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