WB analysis of NIH-3T3 cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30min) treatment using GTX00967 IKB alpha (phospho Ser32) antibody [GT1205]. Dilution : 1:1000 Loading : 25microg
![WB analysis of C6 cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30min) treatment using GTX00967 IKB alpha (phospho Ser32) antibody [GT1205]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX00967/GTX00967_20200327_WB_2_w_23053121_269.webp "WB analysis of C6 cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30min) treatment using GTX00967 IKB alpha (phospho Ser32) antibody [GT1205]. Dilution : 1:1000 Loading : 25microg")
![Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha (phospho Ser32) antibody [GT1205] (GTX00967) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX00967/GTX00967_4000000147_20200313_WB_treatment_TNF-alpha_w_23053121_776.webp "Untreated (-) and treated (+) HeLa whole cell extracts (30 microg) were separated by 10% SDS-PAGE, and the membrane was blotted with IKB alpha (phospho Ser32) antibody [GT1205] (GTX00967) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
WB analysis of NIH-3T3 cell extracts with/without TNF-alpha (20 ng/ml, 37oC, 30min) treatment using GTX00967 IKB alpha (phospho Ser32) antibody [GT1205]. Dilution : 1:1000 Loading : 25microg
IKB alpha (phospho Ser32) antibody [GT1205] - Recombinant antibody, Orthogonal Validated
GTX00967
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNFKBIA
Overview
- SupplierGeneTex
- Product NameIKB alpha (phospho Ser32) antibody [GT1205] - Recombinant antibody, Orthogonal Validated
- Delivery Days Customer9
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDGT1205
- ConjugateUnconjugated
- Gene ID4792
- Target nameNFKBIA
- Target descriptionNFKB inhibitor alpha
- Target synonymsEDAID2; IkappaBalpha; I-kappa-B-alpha; IKBA; ikB-alpha; MAD-3; major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKBI; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
- HostRabbit
- IsotypeIgG
- Modification TypePhosphorylated
- Scientific DescriptionThis gene encodes a member of the NF-kappa-B inhibitor family, which contain multiple ankrin repeat domains. The encoded protein interacts with REL dimers to inhibit NF-kappa-B/REL complexes which are involved in inflammatory responses. The encoded protein moves between the cytoplasm and the nucleus via a nuclear localization signal and CRM1-mediated nuclear export. Mutations in this gene have been found in ectodermal dysplasia anhidrotic with T-cell immunodeficiency autosomal dominant disease. [provided by RefSeq, Aug 2011]
- ReactivityHuman, Mouse, Rat
- Storage Instruction2°C to 8°C,-20°C or -80°C
- UNSPSC12352203