Bio-Connect
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IKB alpha (Ser36) Polyclonal Antibody, Unconjugated (bs-18129R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IKB alpha (Ser36) Polyclonal Antibody, Unconjugated (bs-18129R) at 1:400 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with IKB alpha (Ser36) Polyclonal Antibody, Unconjugated (bs-18129R) at 1:400 overnight at 4°C, DAB staining.

phospho-IKB alpha (Ser36) Polyclonal Antibody

BS-18129R
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNFKBIA
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    IKB alpha (Ser36) Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID4792
  • Target name
    NFKBIA
  • Target description
    NFKB inhibitor alpha
  • Target synonyms
    EDAID2; IkappaBalpha; I-kappa-B-alpha; IKBA; ikB-alpha; MAD-3; major histocompatibility complex enhancer-binding protein MAD3; NF-kappa-B inhibitor alpha; NFKBI; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25963
  • Protein Name
    NF-kappa-B inhibitor alpha
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Src Promotes Metastasis of Human Non-Small Cell Lung Cancer Cells through Fn14-Mediated NF-kappaB Signaling. Wang W et al., 2018 Mar 3, Med Sci Monit
    Read more

Related products

Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetNFKBIA
  • SizePrice
Product group Antibodies
Biosynth
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetNFKBIA
  • SizePrice
Product group Antibodies
Assay Genie
TargetNFKBIA
  • SizePrice
Western Blot analysis of 293 cells using Ikappa;B-alpha Polyclonal Antibody
Product group Antibodies
Cusabio
NFKBIA AntibodyCSB-PA003073
ApplicationsWestern Blot, ELISA
ReactivityHuman, Mouse, Rat
TargetNFKBIA
  • SizePrice
Product group Antibodies
MedChem Express
TargetNFKBIA
  • SizePrice
Immunohistochemical staining of human appendix shows moderate cytoplasmic positivity in lymphoid cells and glandular cells.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetNFKBIA
  • SizePrice
Figure 1. Western blot analysis of IKB Alpha using anti-IKB Alpha antibody (PB9291). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human MDA-MB-453 whole cell lysates, Lane 3: human SW620 whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB Alpha antigen affinity purified polyclonal antibody (Catalog # PB9291) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IKB Alpha at approximately 39KD. The expected band size for IKB Alpha is at 36KD.
Product group Antibodies
Boster Bio
ApplicationsWestern Blot
TargetNFKBIA
  • SizePrice
Product group Antibodies
References
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
TargetNFKBIA
  • SizePrice
Anti-IkB-alpha (N-terminus) Antibody
Product group Antibodies
Antibodies.com
ApplicationsWestern Blot
  • SizePrice