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ProductsBack/IKZF1 Antibody
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate All lanes: IKZF1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 49, 48, 44, 42, 32, 53, 25 kDa Observed band size: 49 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate All lanes: IKZF1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 49, 48, 44, 42, 32, 53, 25 kDa Observed band size: 49 kDa
Western Blot Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate All lanes: IKZF1 antibody at 3.4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 58, 49, 48, 44, 42, 32, 53, 25 kDa Observed band size: 49 kDa

IKZF1 Antibody

CSB-PA621668HA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetIKZF1
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Overview

  • Supplier
    Cusabio
  • Product Name
    IKZF1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10320
  • Target name
    IKZF1
  • Target description
    IKAROS family zinc finger 1
  • Target synonyms
    CLL-associated antigen KW-6; CVID13; DNA-binding protein Ikaros; Hs.54452; IK1; IKAROS; ikaros family zinc finger protein 1; LYF1; LyF-1; lymphoid transcription factor LyF-1; PPP1R92; PRO0758; protein phosphatase 1, regulatory subunit 92; zinc finger protein, subfamily 1A, 1 (Ikaros); ZNFN1A1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13422
  • Protein Name
    DNA-binding protein Ikaros
  • Scientific Description
    Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 4. IHC analysis of Ikaros using anti-Ikaros antibody (PB9643). Ikaros was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 microg/ml rabbit anti-Ikaros Antibody (PB9643) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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