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Raw264.7 lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Raw264.7 lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Raw264.7 lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

IL-1 Beta Polyclonal Antibody

BS-0812R
Bioss Antibodies
ApplicationsWestern Blot
DownloadDatasheet
Product group Antibodies
TargetIL1B
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    IL-1 Beta Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot
  • Applications Supplier
    WB(1:500-2000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3553
  • Target name
    IL1B
  • Target description
    interleukin 1 beta
  • Target synonyms
    catabolin; IL-1; IL-1 beta; IL1beta; IL1-BETA; IL1F2; interleukin 1beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP01584
  • Protein Name
    Interleukin-1 beta
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • A selected small molecule prevents inflammatory osteolysis through restraining osteoclastogenesis by modulating PTEN activity. Chen Y et al., 2020 Dec, Clin Transl Med
    Read more
  • Estrogen deficiency aggravates apical periodontitis by regulating NLRP3/caspase-1/IL-1beta axis. Guan X et al., 2020, Am J Transl Res
    Read more
  • Geniposide alleviates atherosclerosis by regulating macrophage polarization via the FOS/MAPK signaling pathway. Jin Z et al., 2020 May, Biomed Pharmacother
    Read more
  • Glyburide attenuates ozone-induced pulmonary inflammation and injury by blocking the NLRP3 inflammasome. Shao H et al., 2020 Aug, Environ Toxicol
    Read more
  • Dexmedetomidine inhibits the invasion, migration, and inflammation of rheumatoid arthritis fibroblast-like synoviocytes by reducing the expression of NLRC5. Ji YR et al., 2020 Mar 9, Int Immunopharmacol
    Read more
  • Drp1, a potential therapeutic target for Parkinsons disease, is involved in olfactory bulb pathological alteration in the Rotenone-induced rat model. Zhang X et al., 2020 Jun 1, Toxicol Lett
    Read more
  • Resveratrol protects against CIH-induced myocardial injury by targeting Nrf2 and blocking NLRP3 inflammasome activation. Sun ZM et al., 2020 Mar 15, Life Sci
    Read more
  • Anti-Adhesive, Platelet Gathering Effects of c-RGD Modified Poly(p-dioxanone-co-l-Phe) Electrospun Membrane and Its Comprehensive Application in Intestinal Anastomosis. Wang B et al., 2020 Feb, Macromol Biosci
    Read more
  • Effects of Pseudomonas aeruginosa on Microglial-Derived Extracellular Vesicle Biogenesis and Composition. Jones LB et al., 2019 Dec 14, Pathogens
    Read more
  • Is NLRP3 or NLRP6 inflammasome activation associated with inflammation-related lung tumorigenesis induced by benzo(a)pyrene and lipopolysaccharide? Gao M et al., 2019 Dec 15, Ecotoxicol Environ Saf
    Read more

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Figure 1. Western blot analysis of IL-1 beta using anti-IL-1 beta antibody (A00101). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysate, Lane 2: rat spleen tissue lysate, Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 beta antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 beta at approximately 35KD. The expected band size for IL-1 beta is at 31KD.
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