
IL1 beta antibody detects IL1 beta protein by immunofluorescent analysis. Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IL1 beta stained by IL1 beta antibody (GTX100793) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, stained by phalloidin (phalloidin) diluted at 1:100.
IL1 beta antibody
GTX100793
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetIL1B
Overview
- SupplierGeneTex
- Product NameIL1 beta antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3553
- Target nameIL1B
- Target descriptioninterleukin 1 beta
- Target synonymsIL-1, IL1-BETA, IL1F2, IL1beta, interleukin-1 beta, IL-1 beta, catabolin, interleukin 1beta, preinterleukin 1 beta, pro-interleukin-1-beta
- HostRabbit
- IsotypeIgG
- Protein IDP01584
- Protein NameInterleukin-1 beta
- Scientific DescriptionThe protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Zhang C, Chen M, Chi Z. Cytokine secretion and pyroptosis of cholesteatoma keratinocytes mediated by AIM2 inflammasomes in response to cytoplasmic DNA. Mol Med Rep. 2021,23(5):pii: 344. doi: 10.3892/mmr.2021.11983.Read this paper
![IL1 beta antibody [N1C3] detects IL1B protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. IL1 beta antibody [N1C3] (GTX100793) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min IL1 beta antibody [N1C3] detects IL1B protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. IL1 beta antibody [N1C3] (GTX100793) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX100793/GTX100793_39959_20151123_IHC-P_w_23060100_773.webp)
![Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [N1C3] (GTX100793) diluted at 1:500. (WCE: whole cell extract; CM: conditioned medium) Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL1 beta antibody [N1C3] (GTX100793) diluted at 1:500. (WCE: whole cell extract; CM: conditioned medium)](https://www.genetex.com/upload/website/prouct_img/normal/GTX100793/GTX100793_39959_20161222_WB_LPS_ATP_w_23060100_224.webp)




![Sandwich ELISA analysis of human IL-1 beta protein using GTX02972 IL1 beta antibody [MT175] as coating antibody and GTX02971-02 IL1 beta antibody [7P10] (Biotin) as detecting antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02971-02/GTX02971-02_20210507_ELISA_w_23053123_425.webp)
![Sandwich ELISA analysis of human IL-1 beta protein using GTX02972 IL1 beta antibody [MT175] as coating antibody and GTX02971-02 IL1 beta antibody [7P10] (Biotin) as detecting antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX02972/GTX02972_20210507_ELISA_w_23053123_912.webp)
![Sandwich ELISA analysis of human IL-1帣 protein using GTX02972 IL1 beta antibody [MT175] as coating antibody and GTX02971-02 IL1 beta antibody [7P10] (Biotin) as detecting antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX03068/GTX03068_20210507_ELISA_w_23053123_507.webp)
