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Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IL-10 Polyclonal Antibody

BS-0698R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityChicken, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetIl10
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Overview

  • Supplier
    Bioss
  • Product Name
    IL-10 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID16153
  • Target name
    Il10
  • Target description
    interleukin 10
  • Target synonyms
    CSIF, If2a, Il-10, interleukin-10, cytokine synthesis inhibitory factor, interferon 2a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18893
  • Protein Name
    Interleukin-10
  • Reactivity
    Chicken, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Geniposide alleviates atherosclerosis by regulating macrophage polarization via the FOS/MAPK signaling pathway. Jin Z et al., 2020 May, Biomed Pharmacother
    Read this paper
  • A 2D-2D heterojunction Bi2WO6/WS2-x as a broad-spectrum bactericide: Sulfur vacancies mediate the interface interactions between biology and nanomaterials. Hou X et al., 2020 Jun, Biomaterials
    Read this paper
  • Calycosin-7-O-beta-D-glucoside attenuates myocardial ischemia-reperfusion injury by activating JAK2/STAT3 signaling pathway via the regulation of IL-10 secretion in mice. Liu Y et al., 2020 Jan, Mol Cell Biochem
    Read this paper
  • Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway. Wang Y et al., 2019 Jul, Cell Prolif
    Read this paper
  • Tetramethylpyrazine (TMP) ameliorates corneal neovascularization via regulating cell infiltration into cornea after alkali burn. Wu Y et al., 2019 Jan, Biomed Pharmacother
    Read this paper
  • Immune tuning scaffold for the local induction of a pro-regenerative environment. Corradetti B et al., 2017 Dec 5, Sci Rep
    Read this paper
  • LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4. Han S et al., 2017 Jun 22, J Exp Clin Cancer Res
    Read this paper
  • IL35-Producing B Cells Promote the Development of Pancreatic Neoplasia. Pylayeva-Gupta Y et al., 2016 Mar, Cancer Discov
    Read this paper
  • Exposure to 100% Oxygen Abolishes the Impairment of Fracture Healing after Thoracic Trauma. Kemmler J et al., 2015, PLoS One
    Read this paper
  • Hydrogen sulfide reduces kidney injury due to urinary-derived sepsis by inhibiting NF-kappaB expression, decreasing TNF-alpha levels and increasing IL-10 levels. Chen X et al., 2014 Aug, Exp Ther Med
    Read this paper

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Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (A00021). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
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