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Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Paraformaldehyde-fixed, paraffin embedded mouse kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with IL-10 Polyclonal Antibody, Unconjugated (bs-0698R) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.

IL-10 Polyclonal Antibody

BS-0698R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIl10
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Overview

  • Supplier
    Bioss
  • Product Name
    IL-10 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID16153
  • Target name
    Il10
  • Target description
    interleukin 10
  • Target synonyms
    CSIF, If2a, Il-10, interleukin-10, cytokine synthesis inhibitory factor, interferon 2a
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP18893
  • Protein Name
    Interleukin-10
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Jin Z, Li J, Pi J, et al. Geniposide alleviates atherosclerosis by regulating macrophage polarization via the FOS/MAPK signaling pathway. Biomed Pharmacother. 2020,125:110015. doi: 10.1016/j.biopha.2020.110015
    Read this paper
  • Hou X, Shi T, Wei C, et al. A 2D-2D heterojunction Bi(2)WO(6)/WS(2-x) as a broad-spectrum bactericide: Sulfur vacancies mediate the interface interactions between biology and nanomaterials. Biomaterials. 2020,243:119937. doi: 10.1016/j.biomaterials.2020.119937
    Read this paper
  • Liu Y, Che G, Di Z, et al. Calycosin-7-O-β-D-glucoside attenuates myocardial ischemia-reperfusion injury by activating JAK2/STAT3 signaling pathway via the regulation of IL-10 secretion in mice. Mol Cell Biochem. 2020,463(1-2):175-187. doi: 10.1007/s11010-019-03639-z
    Read this paper
  • Wang Y, He G, Tang H, et al. Aspirin inhibits inflammation and scar formation in the injury tendon healing through regulating JNK/STAT-3 signalling pathway. Cell Prolif. 2019,52(4):e12650. doi: 10.1111/cpr.12650
    Read this paper
  • Wu Y, Xu Z, Yang Y, et al. Tetramethylpyrazine (TMP) ameliorates corneal neovascularization via regulating cell infiltration into cornea after alkali burn. Biomed Pharmacother. 2019,109:1041-1051. doi: 10.1016/j.biopha.2018.10.091
    Read this paper
  • Corradetti B, Taraballi F, Corbo C, et al. Immune tuning scaffold for the local induction of a pro-regenerative environment. Sci Rep. 2017,7(1):17030. doi: 10.1038/s41598-017-16895-0
    Read this paper
  • Han S, Wang C, Qin X, et al. LPS alters the immuno-phenotype of glioma and glioma stem-like cells and induces in vivo antitumor immunity via TLR4. J Exp Clin Cancer Res. 2017,36(1):83. doi: 10.1186/s13046-017-0552-y
    Read this paper
  • Pylayeva-Gupta Y, Das S, Handler JS, et al. IL35-Producing B Cells Promote the Development of Pancreatic Neoplasia. Cancer Discov. 2016,6(3):247-55. doi: 10.1158/2159-8290.CD-15-0843
    Read this paper
  • Kemmler J, Bindl R, McCook O, et al. Exposure to 100% Oxygen Abolishes the Impairment of Fracture Healing after Thoracic Trauma. PLoS One. 2015,10(7):e0131194. doi: 10.1371/journal.pone.0131194
    Read this paper
  • Chen X, Xu W, Wang Y, et al. Hydrogen sulfide reduces kidney injury due to urinary-derived sepsis by inhibiting NF-κB expression, decreasing TNF-α levels and increasing IL-10 levels. Exp Ther Med. 2014,8(2):464-470.
    Read this paper

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Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (A00021). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
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