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ProductsBack/FITC Anti-Mouse IL-10 Antibody [JES5-16E3]

FITC Anti-Mouse IL-10 Antibody [JES5-16E3]

AGEL2027
Assay Genie
ApplicationsFlow Cytometry
Product group Antibodies
TargetIl10
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Mouse IL-10 Monoclonal Antibody (FITC Conjugated) [JES5-16E3] (AGEL2027)
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry
  • Applications Supplier
    ICFCM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID16153
  • Target name
    Il10
  • Target description
    interleukin 10
  • Target synonyms
    CSIF; cytokine synthesis inhibitory factor; If2a; IL-; Il-10; interferon 2a; interleukin-10
  • Host
    Rat
  • Protein IDP18893
  • Protein Name
    Interleukin-10
  • Shelf life instruction
    12 months
  • Source
    Rat
  • Reactivity Supplier
    Mouse
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of IL10 using anti-IL10 antibody (A00021). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse brain tissue lysates, Lane 2: mouse spleen tissue lysates, Lane 3: mouse heart tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL10 antigen affinity purified polyclonal antibody (Catalog # A00021) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL10 at approximately 19KD. The expected band size for IL10 is at 21KD.
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