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IL-17RA Antibody (YA2645, Rabbit)

HY-P82900
MedChem Express
TargetIL17RA
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    IL-17RA Antibody (YA2645, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID23765
  • Target name
    IL17RA
  • Target description
    interleukin 17 receptor A
  • Target synonyms
    CANDF5, CD217, CDw217, IL-17RA, IL17R, IMD51, hIL-17R, interleukin-17 receptor A, IL-17 receptor A
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    IL-17RA Antibody (YA2645) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IL-17RA.
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of IL17RA using anti-IL17RA antibody (A01311-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate,Lane 2: human HepG2 whole cell lysate,Lane 3: human MCF-7 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL17RA antigen affinity purified polyclonal antibody (Catalog # A01311-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL17RA at approximately 160KD. The expected band size for IL17RA is at 96KD.
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