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ProductsBack/IL-1B / IL-1 Beta Antibody (clone 3A6)

IL-1B / IL-1 Beta Antibody (clone 3A6)

LS-C169431
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetIL1B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    IL-1B / IL-1 Beta Antibody (clone 3A6)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA (1:10000), IF (1:200 - 1:1000), IHC, IHC-P (1:200 - 1:1000), WB (1:100 - 1:500)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3A6
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3553
  • Target name
    IL1B
  • Target description
    interleukin 1 beta
  • Target synonyms
    catabolin; IL-1; IL-1 beta; IL1beta; IL1-BETA; IL1F2; interleukin 1beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Raw264.7 lysates probed with IL-1 Beta Polyclonal Antibody, Unconjugated (bs-0812R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of IL-1 beta using anti-IL-1 beta antibody (A00101). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human COLO-320 whole cell lysate, Lane 2: rat spleen tissue lysate, Lane 3: mouse NIH3T3 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL-1 beta antigen affinity purified polyclonal antibody (Catalog # A00101) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL-1 beta at approximately 35KD. The expected band size for IL-1 beta is at 31KD.
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